Comparison among Cpp-acp, Fluoride and a Combination of Cpp-acp/ Fluoride and Their Ability to Remineralize White Spot Lesions in Vitro

GUSTAVO OLIVEIRA: Comparison among ACP, Fluoride and a combination of ACP/ Fluoride and their ability to remineralize white spot lesions in vitro (Under the direction of Timothy Wright) This study evaluated the ability of a casein-phosphopeptide amorphous-calcium-phosphate paste (CPP-ACP, MI Paste™), a 1.1% NaF toothpaste containing 5000 ppm of fluoride (ControlRX™) and a CPP-ACP paste with 900 ppm of fluoride (MI Paste Plus™) to remineralize white spot lesions in vitro. Thirty five crowns of sound human molars were sectioned into four specimens. Artificial white spot lesions were formed in all specimens. Specimens were then randomly assigned to one of the following remineralizing treatments (n=35) with a pH-cycling model over 30 days: Group 1 – Control; Group 2 – MIPaste; Group 3-F5000; Group 4 – MI Paste Plus. Therapeutic agents were applied following the manufacturers' directions. MicroCT and Light-induced fluorescence were used as methods of assessment. The NaF containing toothpaste demonstrated better remineralization ability than CPP-ACP products overtime.